DNA fragments generated by the restriction endonucleases in a chemical reaction

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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

Options

(a) Polymerase chain reaction
(b) Electrophoresis
(c) Restriction mapping
(d) Centrifugation

Correct Answer:

Electrophoresis

Explanation:

DNA fragments generated by restriction endonucleases in a chemical reaction can be separated by gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. The DNA fragments separate according to their size through sieving effect provided by matrix.

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Question Type: Memory (964)
Difficulty Level: Easy (1008)
Topics: Biotechnology and Its Applications (123)
Subject: Biology (4253)

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