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Question 1 of 75
1. Question
Which one of the following vectors is used to replace the defective gene in gene therapy?
CorrectIncorrectHint
A virus group which infects the tissue lining of the respiratory tract
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Question 2 of 75
2. Question
Which one of the following is commonly used in transfer of foreign DNA into crop plants?
CorrectIncorrectHint
A bacterium having excellent ability to transfer DNA between itself and plants
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Question 3 of 75
3. Question
There is a restriction endonuclease called EcoRI. What does .co. part in it stand for ?
CorrectIncorrectHint
The organism which is responsible for stomach pain
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Question 4 of 75
4. Question
The linking of antibiotic resistance gene with the plasmid vector became possible with
CorrectIncorrectHint
An enzyme used to join two DNA fragments
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Question 5 of 75
5. Question
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
CorrectIncorrectHint
Sense – antisense
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Question 6 of 75
6. Question
Which one of the following is used as vector for cloning genes into higher organisms?
CorrectIncorrectHint
A RNA containing animal virus
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Question 7 of 75
7. Question
Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin ‘A’ deficiency?
CorrectIncorrectHint
A rice variety which synthesize beta-carotene
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Question 8 of 75
8. Question
Which one of the following techniques made it possible to genetically engineer living organism ?
CorrectIncorrectHint
“Modified genes”
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Question 9 of 75
9. Question
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :
CorrectIncorrectHint
A seperation technique of DNA fragments based on size
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Question 10 of 75
10. Question
The most common substrate used in distilleries for the production of ethanol is
CorrectIncorrectHint
A by product of sugar-making process
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Question 11 of 75
11. Question
A single strand of nucleic acid tagged with a radioactive molecule is called :
CorrectIncorrectHint
A short DNA sequences which is used to detect the presence of complementary DNA sequences by the process called hybridization
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Question 12 of 75
12. Question
A trangenic food crop which may help in solving the problem of night blindness in developing countries is
CorrectIncorrectHint
A rice variety which synthesize beta-carotene
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Question 13 of 75
13. Question
Silencing of mRNA has been used in producing transgenic plants resistant to:
CorrectIncorrectHint
Round worms?
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Question 14 of 75
14. Question
In history of biology, human genome project led to the development of :
CorrectIncorrectHint
Interdisciplinary science field – Combinations of computer science, biology, mathematics and Engineering to interpred the biological data
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Question 15 of 75
15. Question
Which of the following Bt crops is being grown in India by the farmers?
CorrectIncorrectHint
” a thread is from”
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Question 16 of 75
16. Question
PCR and Restriction Fragment Length Polymorphism are the methods for :
CorrectIncorrectHint
DNA Profiling
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Question 17 of 75
17. Question
The genetically-modified (GM) brinjal in India has been developed for:
CorrectIncorrectHint
Hexapoda
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Question 18 of 75
18. Question
Continuous addition of sugars in ‘fed batch’ fermentation is done to:
CorrectIncorrectHint
Use of fed batch fermentation
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Question 19 of 75
19. Question
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :
CorrectIncorrectHint
Ring and bulb are made of
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Question 20 of 75
20. Question
Genes of interest can be selected from a genomic library by using
CorrectIncorrectHint
A short DNA sequences which is used to detect the presence of complementary DNA sequences by the process called hybridization
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Question 21 of 75
21. Question
DNA or RNA segment tagged with a radioactive molecule is called
CorrectIncorrectHint
A fragment of DNA or RNA used in genetic hybridization process
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Question 22 of 75
22. Question
The first clinical gene therapy was given for treating
CorrectIncorrectHint
Inherited disorder which damages the immune system
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Question 23 of 75
23. Question
Maximum number of existing transgenic animals is of :
CorrectIncorrectHint
A small rodent
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Question 24 of 75
24. Question
Agarose extracted from sea weeds finds use in :
CorrectIncorrectHint
A technique to seperate DNA fragments
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Question 25 of 75
25. Question
Polyethylene glycol method is used for
CorrectIncorrectHint
“Direct gene transfer”
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Question 26 of 75
26. Question
Gel electrophoresis is used for
CorrectIncorrectHint
Seperation of DNA based on their size and charge
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Question 27 of 75
27. Question
Bacillus thuringiensis forms protein crystals which contain insecticidal protein.
CorrectIncorrectHint
Insecticidal protein
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Question 28 of 75
28. Question
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bateria because of :
CorrectIncorrectHint
Inactivation of alpha-galactosidase
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Question 29 of 75
29. Question
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
CorrectIncorrectHint
Similar to words “GAAT” Treaty
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Question 30 of 75
30. Question
Main objective of production/use of herbicide resistant GM crops is to
CorrectIncorrectHint
GM crops – tolerant to abiotic stresses
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Question 31 of 75
31. Question
Read the following four statements (A-D) about certain mistakes in two of them
(A)The first transgenic buffalo, Rosie produced milk which was human alpha-lactal albumin enriched.
(B)Restriction enzymes are used in isolation of DNA from other macro-molecules.
(C)Downstream processing is one of the steps of R-DNA technology.
(D)Disarmed pathogen vectors are also used in transfer of R-DNA into the host.
Which are the two statements having mistakes?
CorrectIncorrectHint
The first transgenic buffalo, Rosie and Restriction enzymes related to cut DNA
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Question 32 of 75
32. Question
Which one of the following represents a palindromic sequence in DNA?
CorrectIncorrectHint
“Amma” read in both direction
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Question 33 of 75
33. Question
During the process of isolation of DNA, chilled ethanol is added to
CorrectIncorrectHint
“DNA Clump”
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Question 34 of 75
34. Question
Which one is a true statement regarding DNA polymerase used in PCR
CorrectIncorrectHint
It works even in 90 C
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Question 35 of 75
35. Question
Consider the following statements (A-D) about organic farming:
(A)Utilizes genetically modified crops like Bt cotton
(B)Uses only naturally produced inputs like compost
(C)Does not use pesticides and urea
(D)Produces vegetables rich in vitamins and minerals.
Which of the above statements are correct?CorrectIncorrectHint
Debate is going on GM crops and naturally available compost are used in organic farming
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Question 36 of 75
36. Question
Genetic engineering has been successfully used for producing:
CorrectIncorrectHint
“knockout”
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Question 37 of 75
37. Question
In genetic engineering, the antibiotics are used
CorrectIncorrectHint
GFP, beta-glucoronidase and lacZ are the examples of?
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Question 38 of 75
38. Question
Select the correct statement from the following?
CorrectIncorrectHint
“sewage treatment”
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Question 39 of 75
39. Question
Some of the characteristics of Bt cotton are:
CorrectIncorrectHint
a moth larva which attacks the fruit of cotton
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Question 40 of 75
40. Question
Restriction endonucleases are enzymes which
CorrectIncorrectHint
A molecular scissors
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Question 41 of 75
41. Question
Biolistics (gene-gun) is suitable for
CorrectIncorrectHint
rDNA
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Question 42 of 75
42. Question
Transgenic plants are the ones:
CorrectIncorrectHint
Introduction of genes
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Question 43 of 75
43. Question
What is true about Bt toxin?
CorrectIncorrectHint
Conversion of active from inactive
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Question 44 of 75
44. Question
RNA interference involves
CorrectIncorrectHint
Inhibit gene expression
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Question 45 of 75
45. Question
With the help of DNA ligase donor DNA fragment is joined. It is called
CorrectIncorrectHint
Recombinant DNA
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Question 46 of 75
46. Question
Which one is not biofertilizer
CorrectIncorrectHint
A unique bacterium which is used to control insects
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Question 47 of 75
47. Question
Which is used in production of insulin by genetic engineering
CorrectIncorrectHint
E.coli
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Question 48 of 75
48. Question
Vitamin A rich transgenic plant is
CorrectIncorrectHint
Biosynthesize beta-carotene
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Question 49 of 75
49. Question
Transgenic hirudin is obtained from
CorrectIncorrectHint
The cabbage or mustard family
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Question 50 of 75
50. Question
Tᵢ plasmid transforms cells of
CorrectIncorrectHint
Tree
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Question 51 of 75
51. Question
Thermostable enzymes “Taq and Pfu” isolated from thermophilic bacteria are
CorrectIncorrectHint
Pol I
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Question 52 of 75
52. Question
Thermal cycle is used in
CorrectIncorrectHint
PCR
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Question 53 of 75
53. Question
The technique of production of monoclonal antibodies was developed by
CorrectIncorrectHint
Producing monoclonal antibodies
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Question 54 of 75
54. Question
The Most extensively used bacteria in genetic engineering is
CorrectIncorrectHint
E. coli
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Question 55 of 75
55. Question
The end of DNA fragments are sticky due to
CorrectIncorrectHint
Non-paired bases
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Question 56 of 75
56. Question
Structure involved in genetic engineering is?
CorrectIncorrectHint
Vector
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Question 57 of 75
57. Question
Salt tolerant transgenic has been developed for
CorrectIncorrectHint
Solanum lycopersicum
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Question 58 of 75
58. Question
Restriction enzyme was discovered by
CorrectIncorrectHint
__________ and __________ along with Werner Arber awarded the Nobel Prize in Physiology or MEdicine in the year 1978, For the discovery of restriction enzymes and their application to problems of molecular genetics
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Question 59 of 75
59. Question
Restriction enzyme EcoR I cleavage DNA at the sequence
CorrectIncorrectHint
Palindromic
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Question 60 of 75
60. Question
Restriction endonuclease is employed for cutting
CorrectIncorrectHint
Two polynucleotide chains
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Question 61 of 75
61. Question
Removal and insertion of genes is
CorrectIncorrectHint
Manipulation of an organism’s genes using biotechnology
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Question 62 of 75
62. Question
Recombinant DNA or rDNA technology was discovered by
CorrectIncorrectHint
__________ and __________ worked together to recombine genes. from different bacteria into one DNA molecule.
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Question 63 of 75
63. Question
Plasmids are vectors for gene cloning because they
CorrectIncorrectHint
Presence of Origin of Replication
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Question 64 of 75
64. Question
PCR technique was invented by
CorrectIncorrectHint
In the year 1993 ___________ received a Nobel Prize for his invention of the Polymerase Chain Reaction (PCR) technique
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Question 65 of 75
65. Question
Nucleic acid is fragmented by enzyme
CorrectIncorrectHint
__________ is an enzyme capable of cleaving the phosphodiester bonds between monomers of nucleic acids
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Question 66 of 75
66. Question
Natural genetic engineer is
CorrectIncorrectHint
Agent of crown gall disease
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Question 67 of 75
67. Question
Melting of DNA at 70° C is due to breakdown of
CorrectIncorrectHint
__________ is an electrostatic attraction between two polar groups
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Question 68 of 75
68. Question
Isolation of Bt gene from bacterium Baciullus thuringiensis was undertaken in year
CorrectIncorrectHint
The year in which __________Indira Gandhi returns to power as a result of the mid-term election
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Question 69 of 75
69. Question
In plant biotechnology, root tumours are induced by
CorrectIncorrectHint
____________ is responsible for hairy root disease in a broad range of dicotyledonous plants and some gymnosperms
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Question 70 of 75
70. Question
In genetic engineering, DNA fragments are joined through
CorrectIncorrectHint
Catalyze the joining of two large molecules
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Question 71 of 75
71. Question
In genetic engineering which is used for transfer of genes from one cell to another
CorrectIncorrectHint
Vehicle to artificially carry foreign genetic material
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Question 72 of 75
72. Question
In gel electrophoresis, differential mobility of DNA depends upon
CorrectIncorrectHint
___________ is the technique which is used to seperate macromolecules such as DNA, RNA and Proteins based on their size and charge
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Question 73 of 75
73. Question
Illegal and unlawful development of biomaterials without payment to inhabitants of their origin is called
CorrectIncorrectHint
___________ is the appropriation of another’s knowledge of use of biological resources
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Question 74 of 75
74. Question
Identify the plasmid
CorrectIncorrectHint
The p stands for “plasmid,” and BR for “Bolivar” and “Rodriguez.”
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Question 75 of 75
75. Question
Genomic DNA library is
CorrectIncorrectHint
Stored DNA in vector
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