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The viability of seeds is tested by
Options
(a) Safranine
(b) 2, 6 dichlorophenol indophenols
(c) 2, 3, 5 triphenyl tetrazolium chloride
(d) DMSO
Correct Answer:
2, 3, 5 triphenyl tetrazolium chloride
Explanation:
Dehydrogenase enzyms present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water insoluble compound. This reaction occurs in or near living cells which are releasing hydrogen in respiration processes. Viable tissues produce a normal red-colour, weak living tissue produce an abnormal colour. Dead tissues do not stain, remaining usually white.
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Question Type: Memory
(964)
Difficulty Level: Easy
(1008)
Topics: Reproduction In Plants
(48)
Subject: Biology
(4253)
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