The viability of seeds is tested by

Options

(a) Safranine
(b) 2, 6 dichlorophenol indophenols
(c) 2, 3, 5 triphenyl tetrazolium chloride
(d) DMSO

Correct Answer:

2, 3, 5 triphenyl tetrazolium chloride

Explanation:

Dehydrogenase enzyms present in living tissue reduce the tetrazolium chloride to formazan, a reddish, water insoluble compound. This reaction occurs in or near living cells which are releasing hydrogen in respiration processes. Viable tissues produce a normal red-colour, weak living tissue produce an abnormal colour. Dead tissues do not stain, remaining usually white.