In gel electrophoresis, differential mobility of DNA depends upon

In gel electrophoresis, differential mobility of DNA depends upon

Options

(a) Helical nature of DNA
(b) Double stranded nature of DNA
(c) Charge and size of DNA
(d) Hydrogen bonding between bases

Correct Answer:

Charge and size of DNA

Explanation:

This relationship however breaks down with very large DNA fragments and it is not possible to separate them using standard agarose gel electrophoresis. The limit of resolutiondepends on gel composition and field strength. and the mobility of larger circular DNA may be more strongly affected.

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